A different sequencing method that chemically cleaved DNA at specific bases, developed by Allan Maxam and Walter Gilbert, was the dominant technology into the 1980s. Radiolabeled DNA samples were incubated in four separate reactions, each of which contained a chemical that cleaved after a different nucleotide — either A/G, G, C, or C/T. By adding the right amount of each chemical, it was possible to produce different fragments chopped off at each individual base. The sequence could then be read using gel electrophoresis and autoradiography. Maxam–Gilbert sequencing was easier than the plus and minus method to run and interpret, but was eventually surpassed by Sanger’s chain termination method, which molecular biologists found both technically preferable and more “elegant” since it mirrored the natural copying of DNA.
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8.关于放射性污染防治。第二编第八分编规定了放射性污染防治，分为“一般规定”、“核设施放射性污染防治”、“核技术利用放射性污染防治”、“铀（钍）矿和伴生放射性矿开发利用放射性污染防治”和“放射性废物污染防治”5章。草案在放射性污染防治法的基础上，主要作了以下修改完善：一是明确取得放射性污染监测机构资质的条件（草案第六百一十一条）。二是增加规定国家建立符合受控热核聚变特点、促进核聚变应用的放射性污染防治制度（草案第六百一十三条）。三是完善核设施环评制度（草案第六百一十六条）。四是增加规定放射性同位素和射线装置分级分类管理等制度（草案第六百二十二条等）。五是增加规定伴生放射性矿开发利用辐射环境管理名录等制度（草案第六百三十条等）。六是与原子能法、核安全法等做好衔接，完善放射性废物污染防治制度（草案第六百三十五条等）。